A 100 yr (1911–2011) examination of tick submissions was compiled from the U.S. National Tick Collection and three state databases to determine tick species occurrence in Nebraska. Sixteen tick species were identified including Amblyomma americanum (L.), Dermacentor variabilis (Say), Dermacentor albipictus (Packard), Dermacentor andersoni Stiles, Haemaphysalis leporispalustris (Packard), Rhipicephalus sanguineus (Latreille), and Otobius megnini (Dugès). Amblyomma maculatum Koch and Ixodes scapularis Say were identified in only two and four submissions, respectively, but all identifications have occurred after 1990. County submissions were associated with county population, forested area, and number of recreation areas.