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1 July 2014 A User Friendly Method to Assess Anopheles stephensi (Diptera: Culicidae) Vector Fitness: Fecundity
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Abstract

Fecundity, bloodmeal size, and survival are among the most important parameters in the overall fitness of mosquitoes. Impact of an intervention that affects fecundity can be assessed by directly counting the eggs laid by exposed mosquitoes, which is usually done manually. We have developed a macroinstruction, which can be used to count thousands of Anopheles stephensi Liston eggs in a few minutes, to provide an alternative and adaptable method to egg counting as a measure of fecundity. The macro was developed using a scanner and a computer running AxioVision Rel. 4.8 software, a freely accessible software compatible with Windows XP/7/Vista. Using this semiautomated method, it is possible to reduce time, avoid human error and bias, and obtain improved consistency in studies measuring mosquito fecundity.

© 2014 Entomological Society of America
Edson Dembo, Johnbull Ogboi, Solomon Abay, Giulio Lupidi, Nisha Dahiya, Annette Habluetzel, and Leonardo Lucantoni "A User Friendly Method to Assess Anopheles stephensi (Diptera: Culicidae) Vector Fitness: Fecundity," Journal of Medical Entomology 51(4), 831-836, (1 July 2014). https://doi.org/10.1603/ME13112
Received: 12 June 2013; Accepted: 1 May 2014; Published: 1 July 2014
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