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1 November 2014 The First Molecular Characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) Vitellogenin Expression Products and a Partial cDNA Sequence Isolation
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Abstract

Anopheles (Nyssorhynchus) aquasalis Curry, 1932, is an anopheline that plays an important role in Plasmodium transmission in the New World. Few studies have explored the molecular reproductive biology of this vector species. In the current study, we isolated and sequenced a partial, 886 bp, cDNA fragment of a vitellogenin (Vtg) gene from An. aquasalis. The transcription profile of this Vtg gene in adult females was characterized, and it is similar to other members of the Culicidae. We also detected a smaller subunit of An. aquasalis Vtg proteins in ovary tissue and characterized its translational profile in previtellogenic and vitellogenic females. Our results represent the first molecular characterization of An. aquasalis vitellogenin expression products.

© 2014 Entomological Society of America
André Luis Costa-Da-Silva, Laisa Sentieri Lorenti, Soraia De Lima Oliveira, André Franco Cardoso, and Margareth Lara Capurro "The First Molecular Characterization of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) Vitellogenin Expression Products and a Partial cDNA Sequence Isolation," Journal of Medical Entomology 51(6), 1208-1212, (1 November 2014). https://doi.org/10.1603/ME13045
Received: 1 March 2013; Accepted: 1 August 2014; Published: 1 November 2014
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