Translator Disclaimer
1 November 2015 MtDNA Analysis for Genetic Identification of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) in China
Author Affiliations +
Abstract

DNA-based technologies have been increasingly used in species determination of forensically important sarcophagids, as they are often not morphologically distinct, especially for the immature specimens. The mitochondrial genome has been broadly used for species-level identifications. Although Chinese sarcophagid sequences of short fragments (200–600 bp) had been deposited in GenBank, the barcode region and the complete cytochrome oxidase subunit I (COI) and COII sequences are still unavailable. In this study, 78 sarcophagid fly specimens, representing 17 Chinese sarcophagid species, were collected from 29 locations in 18 Chinese provinces. Sequence data of the mitochondrial COI and COII of the most important Chinese flesh fly taxa associated with cadavers were presented for first time, which serve as reference standards for Chinese species determination. Phylogenetic analysis showed that the COI and COII sequences were useful for identifying most sarcophagid species. The results of this research will be conductive for implementation of the Chinese Sarcophagidae in forensic entomology. However, the application of mitochondrial DNA as species identifier requires great circumspection and additional markers and methods should be studied to ensure accuracy of identification in the future.

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Changquan Zhang, Xiaoliang Fu, Kai Xie, Weitao Yan, and Yadong Guo "MtDNA Analysis for Genetic Identification of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) in China," Journal of Medical Entomology 52(6), 1225-1233, (1 November 2015). https://doi.org/10.1093/jme/tjv131
Received: 20 January 2015; Accepted: 11 August 2015; Published: 1 November 2015
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top