Translator Disclaimer
1 June 2000 IS STAGE CONVERSION THE INITIATING EVENT FOR REACTIVATION OF TOXOPLASMA GONDII IN BRAIN TISSUE OF AIDS PATIENTS?
Author Affiliations +
Abstract

Reactivation of chronic toxoplasmosis resulting in Toxoplasma encephalitis (TE) is a common event in acquired immune deficiency syndrome (AIDS) patients. Conversion from Toxoplasma gondii bradyzoites to tachyzoites is a prerequisite for reactivation. Until recently, the study of stage conversion in human tissue was not possible due to the lack of antibodies that recognize stage-specific epitopes after long-term formaldehyde fixation. Using the combination of a polyclonal anti-T. gondii antibody, the cyst-stage-specific monoclonal antibody CC2, and a tachyzoite-specific polyclonal antibody (anti-SAG1, recombinant), we tried to demonstrate parasite differentiation in the brain tissue of 10 AIDS patients with clinically suspected TE. Double labeling of the stage-specific antibodies enabled us to demonstrate interconversion between tachyzoites and bradyzoites for the first time in human tissue. The study confirmed that the transformation process is nonsynchronous and that the manifestation of TE depends on the degree and site of tissue destruction caused by invading tachyzoites. The original source of tachyzoites could never be located, but a few samples suggested that tachyzoites may invade by dissemination across the blood–brain barrier. Cyst rupture as the first event in the process of reactivation was not seen. We conclude that the initial site(s) of reactivation will be destroyed by tissue-destructive tachyzoites long before clinical symptoms occur.

Ingrid Reiter-Owona, Heike Seitz, Uwe Gross, Monika Sahm, Jürgen K. Rockstroh, and Hanns M. Seitz "IS STAGE CONVERSION THE INITIATING EVENT FOR REACTIVATION OF TOXOPLASMA GONDII IN BRAIN TISSUE OF AIDS PATIENTS?," Journal of Parasitology 86(3), (1 June 2000). https://doi.org/10.1645/0022-3395(2000)086[0531:ISCTIE]2.0.CO;2
Received: 25 May 1999; Accepted: 1 September 1999; Published: 1 June 2000
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top