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1 August 2000 Immunoblot Analysis of Trypomastigote Excreted–Secreted Antigens as a Tool for the Characterization of Trypanosoma cruzi Strains and Isolates
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Abstract

An analysis of antibody recognition of Trypanosoma cruzi exoantigens by immunoblotting revealed a unique banding pattern that seems to be characteristic of each strain or isolate. Trypomastigote excreted–secreted antigens (TESA) present in supernatants of LLC-MK2 cells infected with 5 strains and 10 isolates of T. cruzi produced 13 different immunoblotting patterns. The same bands were observed when probed with acute-phase Chagas' disease serum or with serum from a rabbit immunized with the repetitive domain of T. cruzi transialidase recombinant protein (anti-shed acute-phase antigens). Three similar patterns were observed with TESA from 3 human isolates that probably belong to the same T. cruzi strain. When clone CL Brener, clone CL-14, and CL parental strain were analyzed, the same bands were observed, although they presented different biological behavior. These results suggest that immunoblotting analysis of TESA may be a useful tool for characterization of T. cruzi strains and isolates.

Norival Kesper, Katia A. de Almeida, Anna Maria S. Stolf, and Eufrosina S. Umezawa "Immunoblot Analysis of Trypomastigote Excreted–Secreted Antigens as a Tool for the Characterization of Trypanosoma cruzi Strains and Isolates," Journal of Parasitology 86(4), (1 August 2000). https://doi.org/10.1645/0022-3395(2000)086[0862:IAOTES]2.0.CO;2
Published: 1 August 2000
JOURNAL ARTICLE
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