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1 October 2000 A SCHISTOSOMA MANSONI 62-KDA BAND IS IDENTIFIED AS AN IRRADIATED VACCINE T-CELL ANTIGEN AND CHARACTERIZED AS CALRETICULIN
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Abstract

The Schistosoma mansoni soluble adult worm antigen (SAWA) bands of 62/60 kDa were found to contain immunodominant T-cell immunogen(s) in irradiated cercariae-immunized Swiss and C57BL/6 mice. In the present study, spleen T cells of BALB/c mice immunized twice with ultraviolet light–irradiated cercariae proliferated and produced interleukin 4 in response to the 62/60-kDa SAWA bands in T-cell western assays. To characterize the 62/60-kDa bands, an adult S. mansoni worm cDNA expression library constructed in λgt11 was immunoscreened with serum of mice immunized with the 62/60-kDa antigens, and the immunoreactive cDNA inserts were sequenced. Purified 62- and 60-kDa proteins were used for amino acid microsequencing and for immunization studies in Swiss, C57BL/6, and BALB/c mice and rabbits. Taken together, the data indicated that the 60-kDa molecules are poorly immunogenic in mice and rabbits, whereas the 62-kDa species identified as S. mansoni calreticulin, is a good T- and B-cell antigen and represents a potential vaccine candidate.

Naglaa El Gengehi, Rashika El Ridi, Nagwa Abdel Tawab, Maha El Demellawy, and Beverly L. Mangold "A SCHISTOSOMA MANSONI 62-KDA BAND IS IDENTIFIED AS AN IRRADIATED VACCINE T-CELL ANTIGEN AND CHARACTERIZED AS CALRETICULIN," Journal of Parasitology 86(5), 993-1000, (1 October 2000). https://doi.org/10.1645/0022-3395(2000)086[0993:ASMKBI]2.0.CO;2
Received: 13 August 1999; Accepted: 1 February 2000; Published: 1 October 2000
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