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1 October 2000 SCANT PARASITEMIA IN BALB/C MICE WITH CONGENITAL MALARIA INFECTION
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Abstract

Balb/c mice were examined to determine whether or not they transmitted rodent malaria, Plasmodium berghei, to their fetuses. On the 15th day of pregnancy, mice were inoculated with approximately 3 × 106 P. berghei-infected erythrocytes by peritoneal injection. The blood from 27 adult females and 196 neonates was examined using a sensitive polymerase chain reaction (PCR) method with a detection level of approximately 1 parasite/μl blood. The average parasitemia of females at delivery was 8.1%, ranging from nondetectable to 37.1%. In 12 females, nested PCR established the presence of blood parasite DNA. Malaria parasites were microscopically confirmed in 2 of the 12 neonates. Maternal parasitemia at the time of delivery was not correlated with the incidence of vertical infection (6.1%), which was higher in this study than that found in previous studies. Although the combination of balb/c mice and P. berghei has not been used to examine vertical transmission of malaria, our report showed that this model may be used for this purpose.

Motoi Adachi, Masao Yuda, Katsuhiko Ando, Minoru Sakurai, and Yasuo Chinzei "SCANT PARASITEMIA IN BALB/C MICE WITH CONGENITAL MALARIA INFECTION," Journal of Parasitology 86(5), 1030-1034, (1 October 2000). https://doi.org/10.1645/0022-3395(2000)086[1030:SPIBCM]2.0.CO;2
Received: 23 August 1999; Accepted: 1 February 2000; Published: 1 October 2000
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