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1 April 2001 A Simple Technique to Concentrate the Protozoan Mikrocytos mackini, Causative Agent of Denman Island Disease in Oysters
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Abstract

This report describes a simple filtration technique to isolate the parasite Mikrocytos mackini from oyster tissue. The technique is based on successive filtration through filter papers and polycarbonate membrane filters of decreasing mesh using a low vacuum (<8 cm Hg). This technique allows for the recovery of about 1 × 108 parasites (microcells) from about 2 g of heavily infected oyster tissue. About 99% of the particulate material in the final preparation consisted of intact M. mackini.

J-P. Joly, S. M. Bower, and G. R. Meyer "A Simple Technique to Concentrate the Protozoan Mikrocytos mackini, Causative Agent of Denman Island Disease in Oysters," Journal of Parasitology 87(2), (1 April 2001). https://doi.org/10.1645/0022-3395(2001)087[0432:ASTTCT]2.0.CO;2
Published: 1 April 2001
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