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1 October 2001 EFFECT OF LEISHMANIA DONOVANI LIPOPHOSPHOGLYCAN ON ORNITHINE DECARBOXYLASE ACTIVITY IN MACROPHAGES
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Abstract

Lipophosphoglycan (LPG), a major surface molecule from Leishmania donovani, stimulated ornithine decarboxylase (ODC) activity in macrophages in a dose- and time-dependent manner. LPG stimulated the rapid increase in ODC activity within 30 min after exposure, suggesting that the interaction of LPG with its receptor stimulated a specific signal transduction pathway. However, LPG-induced ODC activity was a transient event because 3 hr after exposure to LPG, no stimulation of ODC activity was detectable. ODC activity appeared to be coupled to the activation of protein kinase C (PKC) in macrophages, as activators of PKC caused a rapid increase in the ODC activity. Macrophages pretreated with LPG for 1 hr became unresponsive to subsequent stimulation by the PKC activators 1-oleoyl-2-acetyl-glycerol and the calcium ionophore A23187. In contrast, the ability of macrophages to express ODC activity in response to the cyclic AMP analogue dibutyryl cyclic AMP was not impaired by LPG.

P. Kapoor, V. Samuel Raj, Shailendra Saxena, Sridevi Balaraman, and Rentala Madhubala "EFFECT OF LEISHMANIA DONOVANI LIPOPHOSPHOGLYCAN ON ORNITHINE DECARBOXYLASE ACTIVITY IN MACROPHAGES," Journal of Parasitology 87(5), (1 October 2001). https://doi.org/10.1645/0022-3395(2001)087[1071:EOLDLO]2.0.CO;2
Received: 19 April 1999; Accepted: 1 April 2001; Published: 1 October 2001
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