Translator Disclaimer
1 December 2001 SALIVARY GLAND EXTRACT FROM IXODES RICINUS TICK POLARIZES THE CYTOKINE PROFILE TOWARD TH2 AND SUPPRESSES PROLIFERATION OF T LYMPHOCYTES IN HUMAN PBMC CULTURE
Author Affiliations +
Abstract

Tick salivary gland extract (SGE) was previously shown to inhibit murine T cell proliferation. In mice, SGE has an inhibitory effect on Th1 and a stimulatory effect on Th2 cytokine elaboration. In the present study, tick-mediated immunomodulation of human T cell proliferation and cytokine elaboration was analyzed using human peripheral blood mononuclear cells (PBMCs) stimulated with concanavalin A (Con A) or lipopolysaccharide (LPS). Using flow cytometry, tick saliva–induced changes were investigated in human mononuclear cell subpopulations. SGE from Ixodes ricinus dose-dependently inhibited human T cell proliferation. This finding supports the flow cytometry data, showing that the percentage of Con A–activated HLA-DR CD3 T lymphocytes and CD4 CD8 double-positive T cells decreased after SGE treatment. SGE significantly inhibited the in vitro production of interleukin-2 (IL-2) and interferon-γ (IFN-γ) secreted by Th1 lymphocytes. In contrast, the elaboration of IL-4, IL-6, and IL-10 secreted by Th2 lymphocytes was significantly stimulated by I. ricinus SGE. Similarly, the production of both IL-1α and IL-1β was significantly stimulated after SGE treatment. These data indicate that the tick-induced immunomodulatory events in humans are similar to those previously described in a murine model.

Lubomír Kovář, Jan Kopecký, and Blanka Říhová "SALIVARY GLAND EXTRACT FROM IXODES RICINUS TICK POLARIZES THE CYTOKINE PROFILE TOWARD TH2 AND SUPPRESSES PROLIFERATION OF T LYMPHOCYTES IN HUMAN PBMC CULTURE," Journal of Parasitology 87(6), 1342-1348, (1 December 2001). https://doi.org/10.1645/0022-3395(2001)087[1342:SGEFIR]2.0.CO;2
Received: 19 January 2001; Accepted: 1 June 2001; Published: 1 December 2001
JOURNAL ARTICLE
7 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top