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1 December 2001 Analysis of a Growth-Promoting Factor for Babesia caballi Cultivation
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Abstract

Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10–20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.

H. Ikadai, M. D. Martin, H. Nagasawa, K. Fujisaki, N. Suzuki, T. Mikami, N. Kudo, T. Oyamada, and I. Igarashi "Analysis of a Growth-Promoting Factor for Babesia caballi Cultivation," Journal of Parasitology 87(6), 1484-1486, (1 December 2001). https://doi.org/10.1645/0022-3395(2001)087[1484:AOAGPF]2.0.CO;2
Published: 1 December 2001
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