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1 December 2001 Isolation of Neospora caninum From the Brain of a Pregnant Sheep
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Abstract

Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction.

T. Koyama, Y. Kobayashi, Y. Omata, M. Yamada, H. Furuoka, R. Maeda, T. Matsui, A. Saito, and T. Mikami "Isolation of Neospora caninum From the Brain of a Pregnant Sheep," Journal of Parasitology 87(6), 1486-1488, (1 December 2001). https://doi.org/10.1645/0022-3395(2001)087[1486:IONCFT]2.0.CO;2
Published: 1 December 2001
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