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1 August 2002 IMMUNO-ELECTRON MICROSCOPIC OBSERVATION OF PLASMODIUM BERGHEI CTRP LOCALIZATION IN THE MIDGUT OF THE VECTOR MOSQUITO ANOPHELES STEPHENSI
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Abstract

The subcellular localization of Plasmodium berghei circumsporozoite protein and thrombospondin-related adhesive protein (PbCTRP) in the invasive stage ookinete of P. berghei was studied in the midgut of Anopheles stephensi by immuno-electron microscopic observations using polyclonal antibodies and immuno-gold labeling. PbCTRP was found to be associated with the micronemes of a mature ookinete throughout the movement from the endoperitrophic space to the basal lamina of the midgut epithelium. PbCTRP was also observed in the electron-dense area outside the ookinete, which might have been secreted from the apical pore. PbCTRP is found most abundantly at the site of contact between the apical end of an ookinete and the basal lamina of an epithelial cell. These results suggest that PbCTRP functions as an adhesion molecule for ookinete movement into the midgut lumen and epithelial cell and for ookinete association with the midgut basal lamina and transformation into an oocyst.

Wutipong Limviroj, Kazuhiko Yano, Masao Yuda, Katsuhiko Ando, and Yasuo Chinzei "IMMUNO-ELECTRON MICROSCOPIC OBSERVATION OF PLASMODIUM BERGHEI CTRP LOCALIZATION IN THE MIDGUT OF THE VECTOR MOSQUITO ANOPHELES STEPHENSI," Journal of Parasitology 88(4), 664-672, (1 August 2002). https://doi.org/10.1645/0022-3395(2002)088[0664:IEMOOP]2.0.CO;2
Received: 1 November 2001; Accepted: 1 January 2002; Published: 1 August 2002
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