Translator Disclaimer
1 December 2002 EXPERIMENTAL INFECTION OF SHEEP WITH NEOSPORA CANINUM OOCYSTS
Author Affiliations +
Abstract

The purpose of the present study was to investigate the potential of Neospora caninum oocysts to infect sheep and determine whether N. caninum DNA could be detected by polymerase chain reaction (PCR) assay in blood and brain of sheep after oocyst inoculation. Six ewes were inoculated per os with 104 N. caninum oocysts, whereas 2 ewes served as uninoculated controls. All sheep were bled weekly for 7 wk after inoculation. Blood was analyzed for the presence of N. caninum DNA by 2 different PCR assays, as well as for the presence of antibodies to recombinant and native N. caninum antigens. Neospora caninum DNA was detected in 2 sheep as early as 7 days after oocyst inoculation (DAOI). All 6 sheep were PCR positive by 32 days and remained positive until the end of the study at 49 DAOI. Aside from 1 ewe, all sheep inoculated with N. caninum oocysts contained detectable N. caninum DNA in the brain tissue collected at 49 DAOI. Unlike with PCR, no lesion or parasite was detected by immunohistochemistry. Antibodies were detected by enzyme-linked immunosorbent assay, Neospora agglutination test, or immunoblotting to either native or recombinant N. caninum antigens in sheep inoculated with oocysts.

Ryan O'Handley, Susan Liddell, Carolyn Parker, Mark C. Jenkins, and J. P. Dubey "EXPERIMENTAL INFECTION OF SHEEP WITH NEOSPORA CANINUM OOCYSTS," Journal of Parasitology 88(6), 1120-1123, (1 December 2002). https://doi.org/10.1645/0022-3395(2002)088[1120:EIOSWN]2.0.CO;2
Received: 7 February 2002; Accepted: 1 June 2002; Published: 1 December 2002
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top