Translator Disclaimer
1 December 2002 A PCR-Based RFLP Analysis of Sarcocystis cruzi (Protozoa: Sarcocystidae) in Yunnan Province, PR China, Reveals the Water Buffalo (Bubalus bubalis) as a Natural Intermediate Host
Author Affiliations +
Abstract

A polymerase chain reaction–based restriction fragment length polymorphism (RFLP) approach is used to examine Sarcocystis cruzi–like taxa from the atypical intermediate host, water buffalo, in Yunnan, People's Republic of China. The loci examined lie within the 18S rRNA gene. A total of 15 water buffalo isolates are compared with those of 10 S. cruzi from cattle. RFLP patterns for the S. cruzi isolates from cattle and the S. cruzi–like taxon from water buffalo are found to be identical with all the 12 restriction enzymes used. Interpopulation variation between samples from Kunming and Gengma (Yunnan) is found to be undetectable at these loci for both S. cruzi and the S. cruzi–like taxon. But RFLPs are found between the S. cruzi taxa and S. suihominis from pigs at the same study sites. These findings support the hypothesis that S. cruzi is able to use the water buffalo as an intermediate host and is not restricted to cattle as was previously supposed.

Qing-Qing Li, Zhao-Qing Yang, Yang-Xian Zuo, S. W. Attwood, Xin-Wen Chen, and Ya-Ping Zhang "A PCR-Based RFLP Analysis of Sarcocystis cruzi (Protozoa: Sarcocystidae) in Yunnan Province, PR China, Reveals the Water Buffalo (Bubalus bubalis) as a Natural Intermediate Host," Journal of Parasitology 88(6), 1259-1261, (1 December 2002). https://doi.org/10.1645/0022-3395(2002)088[1259:APBRAO]2.0.CO;2
Published: 1 December 2002
JOURNAL ARTICLE
3 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top