Autofluorescence of Toxoplasma gondii and Related Coccidian Oocysts

H. D Alan Lindquist; Jason W. Bennett; Jeff D. Hester; Michael W. Ware; J. P. Dubey; William V. Everson

doi:10.1645/GE-3147RN

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H. D Alan Lindquist,¹ Jason W. Bennett,² Jeff D. Hester,³ Michael W. Ware,¹ J. P. Dubey,⁴ William V. Everson¹

¹United States Environmental Protection Agency, 26 W. M.L. King Dr., Cincinnati, Ohio 45268
²Medical College of Ohio, 3000 Arlington Ave., Toledo, Ohio 43614
³Department of Chemistry, University of Cincinnati, Mail Location 0172, Cincinnati, Ohio 45221
⁴Parasitology Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, BARC East Building 1040, Beltsville, Maryland 20705-2350. lindquist.alan@epa.gov

Abstract

This is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of Toxoplasma gondii, Hammondia hammondi, Hammondia heydorni, Neospora caninum, Besnoitia darlingi, and Sarcocystis neurona oocysts or sporocysts. This autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. As with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with an ultraviolet (UV) light source and viewed with the correct UV excitation and emission filter set.

Citation Download Citation

H. D Alan Lindquist, Jason W. Bennett, Jeff D. Hester, Michael W. Ware, J. P. Dubey, and William V. Everson "Autofluorescence of Toxoplasma gondii and Related Coccidian Oocysts," Journal of Parasitology 89(4), 865-867, (1 August 2003). https://doi.org/10.1645/GE-3147RN

Published: 1 August 2003

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