Translator Disclaimer
1 June 2004 CHARACTERIZATION OF PATHOLOGY AND PARASITE LOAD IN OUTBRED AND INBRED MOUSE MODELS OF CHRONIC NEOSPORA CANINUM INFECTION
Author Affiliations +
Abstract
Here, we analyzed histological findings and parasite burden in chronic Neospora caninum infection in BALB/c and ICR mice and studied the correlation between lesion severity and parasite load in brain. To obtain a better understanding of the infection, we examined the influence of various host–pathogen factors. Groups of outbred (ICR) and inbred (BALB/c) mice were inoculated using several NC-1 parasite doses (4 × 105, 106, and 5 × 106 tachyzoites), inoculation routes (intraperitoneal and subcutaneous), and 3 immunosuppressive treatments (methylprednisolone, cyclophosphamide, and vinblastine). Lesion severity was analyzed in the liver, lung, heart, and brain tissues, and parasite load was measured by real-time polymerase chain reaction in brain tissue. The results indicated more severe cerebral lesions and higher brain parasite burdens in inbred than in outbred mice. Hepatic tissue was the primary lesion site in immunosuppressed ICR mice. We also observed that increased inoculum size was reflected in greater lesion severity and a higher cerebral parasite load. No difference was observed with respect to inoculation route. The study also showed an association between brain parasite burden and severity of cerebral lesions in BALB/c mice.
Esther Collantes-Fernández, Gema Álvarez-García, Valentín Pérez-Pérez, Juana Pereira-Bueno and Luis Miguel Ortega-Mora "CHARACTERIZATION OF PATHOLOGY AND PARASITE LOAD IN OUTBRED AND INBRED MOUSE MODELS OF CHRONIC NEOSPORA CANINUM INFECTION," Journal of Parasitology 90(3), (1 June 2004). https://doi.org/10.1645/GE-3290
Received: 14 July 2003; Accepted: 1 March 2004; Published: 1 June 2004
JOURNAL ARTICLE
5 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top