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1 August 2004DEPLETION OF SCHISTOSOMA MANSONI LUNG-STAGE SCHISTOSOMULA CHOLESTEROL BY METHYL-β-CYCLODEXTRIN DRAMATICALLY INCREASES SPECIFIC ANTIBODY BINDING TO SURFACE MEMBRANE ANTIGENS
Schistosoma mansoni lung-stage larvae appear to not bind antibodies from radiation vaccine or infection sera in the membrane immunofluorescence test. However, treatment of ex vivo lung-stage schistosomula with methyl-β-cyclodextrin, a hydrophobic oligosaccharide that specifically extracts cholesterol from plasma membranes, induced readily detectable binding of specific antibodies in a concentration- and time-dependent manner. Surface membrane antigen binding of specific antibodies was also conclusively demonstrated by quantitative absorption of anti-schistosome sera with intact ex vivo larvae. These data together suggest that confinement of lung-stage schistosomula surface membrane antigens in cholesterol-rich sites allows only monovalent antibody binding, which can be detected by absorption and not by direct serology.
"DEPLETION OF SCHISTOSOMA MANSONI LUNG-STAGE SCHISTOSOMULA CHOLESTEROL BY METHYL-β-CYCLODEXTRIN DRAMATICALLY INCREASES SPECIFIC ANTIBODY BINDING TO SURFACE MEMBRANE ANTIGENS," 90(4) Rashika El Ridi, Hatem Tallima, Shadia H. Mohamed, Mona Montash Journal of Parasitology (1 August 2004)
