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1 August 2004 DEPLETION OF SCHISTOSOMA MANSONI LUNG-STAGE SCHISTOSOMULA CHOLESTEROL BY METHYL-β-CYCLODEXTRIN DRAMATICALLY INCREASES SPECIFIC ANTIBODY BINDING TO SURFACE MEMBRANE ANTIGENS
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Abstract
Schistosoma mansoni lung-stage larvae appear to not bind antibodies from radiation vaccine or infection sera in the membrane immunofluorescence test. However, treatment of ex vivo lung-stage schistosomula with methyl-β-cyclodextrin, a hydrophobic oligosaccharide that specifically extracts cholesterol from plasma membranes, induced readily detectable binding of specific antibodies in a concentration- and time-dependent manner. Surface membrane antigen binding of specific antibodies was also conclusively demonstrated by quantitative absorption of anti-schistosome sera with intact ex vivo larvae. These data together suggest that confinement of lung-stage schistosomula surface membrane antigens in cholesterol-rich sites allows only monovalent antibody binding, which can be detected by absorption and not by direct serology.
Rashika El Ridi, Hatem Tallima, Shadia H. Mohamed and Mona Montash "DEPLETION OF SCHISTOSOMA MANSONI LUNG-STAGE SCHISTOSOMULA CHOLESTEROL BY METHYL-β-CYCLODEXTRIN DRAMATICALLY INCREASES SPECIFIC ANTIBODY BINDING TO SURFACE MEMBRANE ANTIGENS," Journal of Parasitology 90(4), (1 August 2004). https://doi.org/10.1645/GE-3334
Received: 14 October 2003; Accepted: 1 January 2004; Published: 1 August 2004
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