Translator Disclaimer
1 February 2005 Detection of Antibodies to Neospora caninum in Cattle by Enzyme-Linked Immunosorbent Assay with Truncated NcSRS2 Expressed in Escherichia coli
Author Affiliations +
Abstract

The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal signal peptide and C-terminal hydrophobic regions was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. ELISA could discriminate clearly between known N. caninum–positive and –negative sera from cattle. Field serum samples collected from cattle in Brazil were examined for the diagnosis of N. caninum infection using ELISA. Of the 197 samples analyzed, 64 (32.5%) samples were positive for antibodies to N. caninum. Of the 64 ELISA-positive samples, 58 (90.6%) were confirmed as positive by Western blot analysis with whole-parasite antigens. These results suggest that ELISA with recombinant NcSRS2t is an effective method for diagnosis of N. caninum infection in cattle.

Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, Haruyuki Hirata, Yoshihumi Nishikawa, Yasuhiro Takashima, Hiroshi Suzuki, Kozo Fujisaki, and Chihiro Sugimoto "Detection of Antibodies to Neospora caninum in Cattle by Enzyme-Linked Immunosorbent Assay with Truncated NcSRS2 Expressed in Escherichia coli," Journal of Parasitology 91(1), (1 February 2005). https://doi.org/10.1645/GE-267R1
Published: 1 February 2005
JOURNAL ARTICLE
2 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top