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1 February 2005 A SPECIFIC PRIMER PAIR FOR THE DIAGNOSIS AND IDENTIFICATION OF ACANTHAMOEBA ASTRONYXIS BY RANDOM AMPLIFIED POLYMORPHIC DNA– POLYMERASE CHAIN REACTION
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Abstract

Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using the OPC20 primer (ACTTCGCCAC). The band specificity was confirmed by hybridization, using it as a probe, against all OPC20 amplifications from different Acanthamoeba species. Once the fragment was sequenced, we used it to design a specific primer pair that was useful for the identification of different isolates as A. astronyxis species.

A. Ortega-Rivas, J. Lorenzo-Morales, E. Martínez, M. Villa, A. Clavel, B. Valladares, and A. del Castillo "A SPECIFIC PRIMER PAIR FOR THE DIAGNOSIS AND IDENTIFICATION OF ACANTHAMOEBA ASTRONYXIS BY RANDOM AMPLIFIED POLYMORPHIC DNA– POLYMERASE CHAIN REACTION," Journal of Parasitology 91(1), 122-126, (1 February 2005). https://doi.org/10.1645/GE-3301
Received: 6 August 2003; Accepted: 1 June 2004; Published: 1 February 2005
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