Translator Disclaimer
1 February 2005 Detection of Paragonimus heterotremus Eggs in Experimentally Infected Cats by a Polymerase Chain Reaction–Based Method
Author Affiliations +
Abstract

A polymerase chain reaction (PCR) procedure for the detection of Paragonimus heterotremus eggs in stool samples was developed and compared with Stoll's egg count method. The primers were designed on the basis of a previously constructed pPH-13–specific DNA probe, which produced an approximate 0.5-kb amplified product. This PCR method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 × 10−4 ng of P. heterotremus genomic DNA. The assay had 100% sensitivity in all infected cats. The method did not yield an approximate 0.5-kb product with DNA from other parasites such as Gnathostoma spinigerum, Trichinella spiralis, Fasciola gigantica, Echinostoma malayanum, Opisthorchis viverrini, Dirofilaria immitis, and Taenia saginata; exceptions were Paragonimus siamensis and Paragonimus westermani. In addition, no genomic DNA from Escherichia coli, Burkholderia pseudomallei, Acinetobacter anitratus, Mycobacterium tuberculosis, Staphylococcus aureus, β-Streptococcus grA, and Proteus mirabilis or from the vertebrate and invertebrate hosts of P. heterotremus was amplified in the PCR assay. This assay has great potential for application in clinical epidemiological studies.

Pewpan M. Intapan, Chaisiri Wongkham, Kanokwan J. Imtawil, Wilawan Pumidonming, Thidarat K. Prasongdee, Masanao Miwa, and Wanchai Maleewong "Detection of Paragonimus heterotremus Eggs in Experimentally Infected Cats by a Polymerase Chain Reaction–Based Method," Journal of Parasitology 91(1), 195-198, (1 February 2005). https://doi.org/10.1645/GE-3357RM
Published: 1 February 2005
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top