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1 February 2005 PROTECTIVE EFFECT OF ANTIGANGLIOSIDE ANTIBODIES AGAINST EXPERIMENTAL TRYPANOSOMA BRUCEI INFECTION IN MICE
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Abstract

Liposome-associated ganglioside antigens (ganglioside GM1 or bovine brain gangliosides) were prepared to facilitate the potential protective efficacy for Trypanosoma brucei. Mice were immunized with liposome-associated ganglioside GM1 or bovine brain gangliosides intraperitoneally (i.p.). After immunization, significantly higher antigen-specific IgG and IgM antibodies were detected in sera than in the nonimmunized control group. When sera from immunized mice were analyzed for isotype distribution, antigen-specific IgG1, IgG2a, and IgG3 antibody responses were also noted. After immunization, mice were challenged i.p. with 1 × 102 cells of T. brucei. Sixty percentage of liposome-associated ganglioside GM1–immunized mice survived the infection, and all the mice immunized with bovine brain gangliosides–containing liposomes survived. However, all control mice died within 7 days after infection. These data demonstrate that liposomes containing ganglioside antigens have the potential usefulness for the induction of a protective immune response against T. brucei infection and suggest the possibility of developing vaccines that may ultimately be used for the prevention of trypanosomiasis.

Akiko Uemura, Shinobu Watarai, Yoshihiro Ohnishi, and Hiroshi Kodama "PROTECTIVE EFFECT OF ANTIGANGLIOSIDE ANTIBODIES AGAINST EXPERIMENTAL TRYPANOSOMA BRUCEI INFECTION IN MICE," Journal of Parasitology 91(1), 73-78, (1 February 2005). https://doi.org/10.1645/GE-3375
Received: 30 December 2003; Accepted: 1 May 2004; Published: 1 February 2005
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