Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and Evaluation of its Protective Effects

Hiroaki Shirafuji; Xuenan Xuan; Isao Kimata; Yasuhiro Takashima; Shinya Fukumoto; Haruki Otsuka; Hideyuki Nagasawa; Hiroshi Suzuki

doi:10.1645/GE-364R1

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Hiroaki Shirafuji,¹ Xuenan Xuan,^1,2 Isao Kimata,^1,3 Yasuhiro Takashima,^1,4 Shinya Fukumoto,¹ Haruki Otsuka,¹ Hideyuki Nagasawa,¹ Hiroshi Suzuki¹

¹National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
²To whom correspondence should be addressed
³Department of Medical Zoology, Faculty of Medicine, Osaka City University, Abeno-ku, Osaka 545-8585, Japan
⁴Department of Global Agricultural Science, Graduate School of Agriculture and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. gen@obihiro.ac.jp

Abstract

In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse model. The molecular weight and antigenic property of P23 expressed by Tg/P23 were similar to those of the native P23. Mice immunized with lysed Tg/P23 tachyzoites produced specific neutralizing antibodies against C. parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals.

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Hiroaki Shirafuji, Xuenan Xuan, Isao Kimata, Yasuhiro Takashima, Shinya Fukumoto, Haruki Otsuka, Hideyuki Nagasawa, and Hiroshi Suzuki "Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and Evaluation of its Protective Effects," Journal of Parasitology 91(2), 476-479, (1 April 2005). https://doi.org/10.1645/GE-364R1

Published: 1 April 2005

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