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1 April 2005 Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites
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Abstract

Mechanisms of host immunosuppression after infection with Toxoplasma gondii are unclear. This study was performed to observe cytokine and immunoglobulin secretions by murine splenic lymphocytes infected in vitro with live, nonreplicating (irradiated) RH tachyzoites on stimulation with concanavalin A (Con A) or lipopolysaccharide (LPS). For lymphocyte cultivation, 3 groups were prepared: coculture with live nonirradiated tachyzoites separated by a transwell (group T), live irradiated tachyzoites without a transwell (group R), and no tachyzoites (group C). Compared with group T, groups R and C, on stimulation with Con A, revealed significantly (P < 0.05) lower levels of interleukin-2 (IL-2) and IFN-γ, but not IL-10. The levels of IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM were also significantly (P < 0.05) lower in groups R and C than in group T after stimulation with LPS. The results suggest that intracellular infection of murine splenic lymphocytes with T. gondii tachyzoites could impair their capacity to produce cytokine and immunoglobulin secretions.

S-M. Guk, J. Kook, Y-H. Jeon, J-H. Choi, E-T. Han, E-H. Shin, and J-Y. Chai "Suppressed Cytokine and Immunoglobulin Secretions by Murine Splenic Lymphocytes Infected In Vitro with Toxoplasma gondii Tachyzoites," Journal of Parasitology 91(2), 467-470, (1 April 2005). https://doi.org/10.1645/GE-404R
Published: 1 April 2005
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