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1 June 2005 A Restriction Enzyme–Based Assay to Distinguish Between Avian Hemosporidians
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Abstract
We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leucocytozoon. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full mitochondrial genomes from two Haemoproteus and three Plasmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemias of approximately 8 × 10−6 per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.
Jon S. Beadell and Robert C. Fleischer "A Restriction Enzyme–Based Assay to Distinguish Between Avian Hemosporidians," Journal of Parasitology 91(3), (1 June 2005). https://doi.org/10.1645/GE-3412RN
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