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1 October 2005 NAD(P)H-OXIDASE PRESENCE IN TOXOPLASMA GONDII TACHYZOITE VACUOLE DURING INTERACTION WITH IFN-GAMMA–ACTIVATED HUMAN ENDOTHELIAL CELLS
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Abstract

Toxoplasma gondii invades and proliferates in human umbilical vein endothelial cells (HUVEC) where it resides in a parasitophorous vacuole (PV) preventing lysosomal fusion. To study the intracellular outcome of PV containing tachyzoites of T. gondii during interaction with IFN-γ–activated HUVEC, a quantitative analysis of the T. gondii infection and multiplication was assayed. The quantification of PVs' fusion with lysosomes, ultrastructural examination of phagosome–lysosome fusion, and the localization of NAD(P)H-oxidase activity were also investigated. HUVEC activated with IFN-γ inhibited T. gondii infection and multiplication by 67.5% and 91.0%, respectively. After 4 hr of infection, 10.2% of IFN-γ–activated HUVEC exhibited phagosome–lysosome fusion assayed by fluorescence microscopy, which was also observed at the ultrastructural level. Furthermore, the enzyme NAD(P)H-oxidase present at the plasma membrane of activated HUVEC was internalized together with the parasite in 38.0% of the cells. In addition, colocalization of colloidal gold particles and reaction product of NAD(P)H-oxidase in the PV of some activated HUVEC was observed. These results suggest that NAD(P)H-oxidase may participate in a mechanism by which IFN-γ–activated HUVEC inhibit T. gondii multiplication.

Erika Cortez, Ana Carolina Stumbo, Técia Maria U. de Carvalho, Helene Santos Barbosa, and Laís Carvalho "NAD(P)H-OXIDASE PRESENCE IN TOXOPLASMA GONDII TACHYZOITE VACUOLE DURING INTERACTION WITH IFN-GAMMA–ACTIVATED HUMAN ENDOTHELIAL CELLS," Journal of Parasitology 91(5), 1052-1057, (1 October 2005). https://doi.org/10.1645/GE-467R1.1
Received: 23 September 2004; Accepted: 1 March 2005; Published: 1 October 2005
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