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1 December 2005 DIFFERENTIAL IDENTIFICATION OF ASCOGREGARINA SPECIES (APICOMPLEXA: LECUDINIDAE) IN AEDES AEGYPTI AND AEDES ALBOPICTUS (DIPTERA: CULICIDAE) BY POLYMERASE CHAIN REACTION
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Abstract

We report 2 polymerase chain reaction (PCR)-based methods for distinguishing morphologically similar gregarine species based on amplification of variable regions of the internal transcribed spacer region of ribosomal DNA. The gregarines we investigated were Ascogregarina barretti (Vavra), A. culicis (Ross), and A. taiwanensis (Lien and Levine), parasites of the mosquitoes Ochlerotatus triseriatus (Say), Aedes aegypti (Linnaeus), and Ae. albopictus (Skuse), respectively. These 3 important vector mosquitoes often utilize the same container habitats, where larval development and infection by the parasite occurs, leaving ample opportunity for cross-species gregarine infection. Because previous studies have shown that the parasites A. culicis and A. taiwanensis variably affect fitness in both normal and abnormal mosquito hosts, distinguishing parasite infection and species is important. The task is complicated by the fact that these 2 parasite species are virtually identical in morphology, whereas A. barretti is morphologically distinct. Of the 2 PCR-based assays reported here, the first provides a rapid, sensitive, and straightforward means of general ascogregarine detection based on a single PCR amplification. The second method provides a means of differentiation between A. culicis and A. taiwanensis based on a species-specific PCR assay. Together, these assays allow whole mosquitoes to be tested for the presence of Ascogregarina species as well as identification of both A. culicis and A. taiwanensis singly or in dual infections.

Maria E. Morales, Clara B. Ocampo, Horacio Cadena, Claudia S. Copeland, Michael Termini, and Dawn M. Wesson "DIFFERENTIAL IDENTIFICATION OF ASCOGREGARINA SPECIES (APICOMPLEXA: LECUDINIDAE) IN AEDES AEGYPTI AND AEDES ALBOPICTUS (DIPTERA: CULICIDAE) BY POLYMERASE CHAIN REACTION," Journal of Parasitology 91(6), (1 December 2005). https://doi.org/10.1645/GE-442R.1
Received: 29 July 2004; Accepted: 8 March 2005; Published: 1 December 2005
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