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1 February 2006 CHARACTERIZATION OF TOXOPLASMA GONDII ISOLATES IN FREE-RANGE CHICKENS FROM AMAZON, BRAZIL
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Abstract

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii in 50 free-range chickens (Gallus domesticus) from Amazon, Brazil, was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 33 (66%) chickens with titers of 1:5 in 3, 1:10 in 2, 1:20 in 1, 1:40 in 1, 1:80 in 2, 1:160 in 5, 1:200 in 9, 1:400 in 5, 1:800 in 2, 1:1,600 in 2, and 1:3,200 or higher in 1. Hearts and brains of 33 seropositive chickens were bioassayed individually in mice. Tissues from 17 seronegative chickens were pooled and fed to 2 T. gondii–free cats. Feces of cats were examined for oocysts, but none was found. Toxoplasma gondii was isolated from 24 chickens with MAT titers of 1:5 or higher. Genotyping of these 24 T. gondii isolates by polymorphisms at the SAG2 locus indicated that 14 were type I, and 10 were type III; the absence of type II strains from Brazil was confirmed. Fifty percent of the infected mice died of toxoplasmosis, irrespective of the genotype.

J. P. Dubey, Solange M. Gennari, Marcelo B. Labruna, Luis M. A. Camargo, M. C B. Vianna, Paula L. Marcet, and T. Lehmann "CHARACTERIZATION OF TOXOPLASMA GONDII ISOLATES IN FREE-RANGE CHICKENS FROM AMAZON, BRAZIL," Journal of Parasitology 92(1), (1 February 2006). https://doi.org/10.1645/GE-655R.1
Received: 15 June 2005; Accepted: 1 July 2005; Published: 1 February 2006
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