Translator Disclaimer
1 April 2006 EFFECTS OF POLYAMINES ON TWO STRAINS OF TRYPANOSOMA BRUCEI IN INFECTED RATS AND IN VITRO CULTURE
Author Affiliations +
Abstract

We studied the effects of polyamines, which are necessary for proliferation and antioxidation in Trypanosoma brucei gambiense Wellcome strain (WS) and Trypanosoma brucei brucei ILtat 1.4 strain (IL). No difference was found in activity of ornithine decarboxylase (ODC), a key enzyme in polyamine synthesis in trypanosomes, in both strains maintained in vitro; higher (P < 0.05) ODC values were found in IL in vivo. However, WS in vivo exhibited higher proliferation rates with higher spermidine content and decreased host survival times than IL. The in vitro proliferation and polyamine contents of WS increased with the addition of polyamine to the l-difluoromethylornithine culture medium, but not IL. These results suggested that WS uses extracellular polyamine for proliferation. In the in vitro culture, WS was less tolerant of hydrogen peroxide (oxidative stress) than IL, and malondialdehyde levels in WS were higher than in IL. The expression of trypanothione synthetase mRNA in WS in vitro was higher than in IL. These results suggest that IL is dependent on the synthesis of polyamines for proliferation and reduction of oxidative stress, whereas WS is dependent on the uptake of extracellular polyamines. A thorough understanding of the differences in the metabolic capabilities of various trypanosomes is important for the design of more effective medical treatments.

Kazuhiko Nishimura, Takako Yanase, Noriko Araki, Yoshihiro Ohnishi, Shunji Kozaki, Kensuke Shima, Masahiro Asakura, Worada Samosomsuk, and Shinji Yamasaki "EFFECTS OF POLYAMINES ON TWO STRAINS OF TRYPANOSOMA BRUCEI IN INFECTED RATS AND IN VITRO CULTURE," Journal of Parasitology 92(2), (1 April 2006). https://doi.org/10.1645/GE-633R.1
Received: 2 May 2005; Accepted: 1 July 2005; Published: 1 April 2006
JOURNAL ARTICLE
7 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top