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1 June 2007 DNA Probes for Identifying Chromosomes 5, 6, and 7 of Schistosoma mansoni
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Abstract

Schistosoma mansoni has a genome of 270 Mb contained on 8 pairs of chromosomes. C-banding has been a useful technique in identifying the 7 autosomal and sex chromosomes. However, even with C-banding, S. mansoni chromosomes 5, 6, and 7 are difficult to discriminate from each other, because of their small sizes, morphological similarity, and poor banding patterns. We have identified probes that specifically paint chromosomes 5, 6, and 7 of S. mansoni with the use of chromosome microdissection and the degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). Exact chromosome identification is required for accurate chromosome mapping of genomic clones and genetic elements, which is an essential component of the schistosome genome project.

T. Taguchi, Y. Hirai, P. T. LoVerde, A. Tominaga, and H. Hirai "DNA Probes for Identifying Chromosomes 5, 6, and 7 of Schistosoma mansoni," Journal of Parasitology 93(3), 724-726, (1 June 2007). https://doi.org/10.1645/GE-1099R.1
Published: 1 June 2007
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