Observations on Cryptosporidium Life Cycle Stages During Excystation

Panagiotis Karanis; Akio Kimura; Hideyuki Nagasawa; Ikuo Igarashi; Naoyoshi Suzuki

doi:10.1645/GE-1185.1

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Panagiotis Karanis,¹ Akio Kimura,² Hideyuki Nagasawa,³ Ikuo Igarashi,³ Naoyoshi Suzuki³

¹Medical and Molecular Parasitology Laboratory, Medical School, Center of Anatomy, Institute II, University of Cologne, 50937 Cologne, Germany
²Osaka Prefectural Institute of Public Health, Department of Virology, Osaka 537-0025, Japan
³National Research Center for Protozoan Diseases, Obihiro University for Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. karanis@obihiro.ac.jp

Abstract

Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly spindle-shaped; others were bean shaped, actively motile, and underwent division. Merozoites survived for short time–period in an in vitro culture system, but could not be established in a subsequent cultivation effort in RPMI medium.

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Panagiotis Karanis, Akio Kimura, Hideyuki Nagasawa, Ikuo Igarashi, and Naoyoshi Suzuki "Observations on Cryptosporidium Life Cycle Stages During Excystation," Journal of Parasitology 94(1), 298-300, (1 February 2008). https://doi.org/10.1645/GE-1185.1

Published: 1 February 2008

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