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1 June 2009 A Novel Taenia solium Protein That Resembles Troponin T Proteins
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Taenia solium Linnaeus, 1758 is responsible for taeniasis and cysticercosis, which are 2 serious health problems, particularly in developing countries. The attempt to identify a 22.5kD possible protective oncospheral antigen by 2-dimensional gel-electrophoresis, micro-sequencing, and cDNA library screening produced a protein of 42kD that possesses a conserved domain similar to that of troponin T. Five variants that showed differences at the 5′ end were observed at the cDNA level. Hyper-immune rabbit sera developed against recombinant GST fused protein identified the protein exclusively on activated oncospheres. The 42kD protein was tested in an enzyme-linked immunoassay (ELISA) alone and then together with the Tso31 protein for the diagnosis of human cysticercosis. When both antigens were combined, the test was found to be 85% sensitive and 65% specific. The 42kD is a novel T. solium protein that is present exclusively on activated oncospheres of this parasite, with poor diagnostic activity against taeniasis or human cysticercosis.

Holger Mayta, Kathy Hancock, Robert H. Gilman, Roxana Zamudio, Janeth P. Castillo, Min Z. Levine, Hector H. Garcia, Armando E. Gonzalez, and Victor C. W. Tsang "A Novel Taenia solium Protein That Resembles Troponin T Proteins," Journal of Parasitology 95(3), 591-597, (1 June 2009).
Received: 1 July 2008; Accepted: 1 December 2008; Published: 1 June 2009

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