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1 June 2009 Cd4 T-cell-dependent Goblet Cell Proliferation and Expulsion of Gymnophalloides seoi from the Intestine of C57bl/6 Mice
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Abstract

Mechanisms for the spontaneous worm expulsion from the host intestine are not well understood in gastrointestinal trematode models. We studied the role of CD4 T-helper cells in mediating goblet cell hyperplasia and expulsion of Gymnophalloides seoi from the intestines of C57BL/6 (resistant) and ICR (susceptible) mice. C57BL/6 mice expelled all G. seoi worms within 4 days post-infection (PI), while ICR mice did not completely expel worms until day 7 PI. This difference in worm expulsion was associated with high numbers of mucosal goblet cells in C57BL/6 mice along with alteration of the mucin quality, with changes in the terminal sugar chain and high levels of IL-4 and IL-5 mRNA expression in mesenteric lymph nodes. Adoptive transfer of mucosal CD4 T-helper cells to syngeneic mice elicited strong goblet cell hyperplasia and a notably accelerated worm expulsion. However, this T-helper cell transfer had no relationship with the alteration of mucin quality. The results showed that CD4 T-helper cells play an important role as a mediator of goblet cell hyperplasia, but not for functional activation of goblet cells. It is suggested that both T-cell dependent and independent mechanisms operate for expulsion of G. seoi from the mouse intestine.

Sang-Mee Guk, Jun-Haeng Lee, Hyo-Jin Kim, Won-Hee Kim, Eun-Hee Shin, and Jong-Yil Chai "Cd4 T-cell-dependent Goblet Cell Proliferation and Expulsion of Gymnophalloides seoi from the Intestine of C57bl/6 Mice," Journal of Parasitology 95(3), 581-590, (1 June 2009). https://doi.org/10.1645/GE-1807.1
Received: 31 July 2008; Accepted: 1 November 2008; Published: 1 June 2009
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