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1 December 2009 Identification of Antigenic Brugia Adult Worm Proteins by Peptide Mass Fingerprinting
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Abstract
With the recent completion of the Brugia malayi genome, proteomics offers a new resource for a deeper understanding of the biology of filarial parasites. We employed 2-dimensional (2D) gel electrophoresis followed by peptide mass fingerprinting on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometer to identify Brugia adult worm proteins and then determined which proteins were recognized by the host humoral immune response. We identified 18 unique proteins, several of which were determined to be antigenic by immunoblot. The proteins identified here may contribute to future studies to analyze the transmission and pathogenesis of lymphatic filariasis.
Tiffany Weinkopff, James A. Atwood, George A. Punkosdy, Delynn Moss, D. Brent Weatherly, Ron Orlando and Patrick Lammie "Identification of Antigenic Brugia Adult Worm Proteins by Peptide Mass Fingerprinting," Journal of Parasitology 95(6), (1 December 2009). https://doi.org/10.1645/GE-2083.1
Received: 3 March 2009; Accepted: 1 June 2009; Published: 1 December 2009
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