Gyrodactylus granoei n. sp. (Monogenea: Gyrodactylidae) is described from the fins and body surface of the spine loach, Cobitis granoei (Rendahl) (Cobitidae), in central China. It resembles a suite of species from cyprinid and cobitid fishes that have short, compact hamuli, a ventral bar with no obvious anterolateral projections, a linguiform posterior membrane, a male copulatory organ with small hooks in multiple rows, a simple cylindrical dorsal bar, and short marginal hooks, with an expanded sickle heel. Of these, the new species resembles most closely Gyrodactylus micracanthus Hukuda, 1940 from Misgurnus anguillicaudatus (Cantor) (Cobitidae), but it is identified by the length of the hamuli and the morphology of dorsal and ventral bars. When searched using BLAST, sequence data (829 bp) spanning the ITS1, 5.8s, and ITS2 region did not return an identical match; close similarity (82–88%) was found with sequenced members of the subgenus Gyrodactylus. It is suggested that the new species is part of a freshwater lineage that has radiated successfully among cyprinid fishes in North America, Europe, and Asia, and some of their predator and amphibian neighbors.