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1 October 2010 Intragenomic Sequence Variation of the ITS-1 Region Within a Single Flow-Cytometry–Counted Cyclospora cayetanensis Oocysts
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Abstract

Cyclospora cayetanensis, a protozoan of emerging concern, causes self-limiting gastroenteritis in immune-competent hosts. It has been established that sequence variability exists in the first internal transcribed spacer region (ITS-1) of the ribosomal DNA operon from collections of oocysts obtained from individual or pooled fecal samples. To determine if single oocysts also exhibited ITS-1 sequence variability, DNA was extracted from individually flow-cytometry–counted oocysts. We determined that ITS-1 sequence variability exists at an individual-genome level for C. cayetanensis and approached or exceeded the variability exhibited among oocyst collections. ITS-1 variability, at the genome level, reduces this region's utility for inferring relationships between strains.

Diana K. Riner, Tonya Nichols, Sasha Y. Lucas, Andrew S. Mullin, John H. Cross, and H. D. Alan Lindquist "Intragenomic Sequence Variation of the ITS-1 Region Within a Single Flow-Cytometry–Counted Cyclospora cayetanensis Oocysts," Journal of Parasitology 96(5), 914-919, (1 October 2010). https://doi.org/10.1645/GE-2505.1
Received: 5 April 2010; Accepted: 1 May 2010; Published: 1 October 2010
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