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1 February 2011 Polymorphism Analysis of Chinese Theileria sergenti Using Allele-Specific Polymerase Chain Reaction of the Major Piroplasm Surface Protein Gene
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Abstract

Theileria sergenti is a tick-borne parasite found in many parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a marker for epidemiological and phylogenetic studies of benign Theileria species. In this study, Chinese species of T. sergenti were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the MPSP gene. Using universal or allele-specific primer sets for PCR amplification of the MPSP gene, 98 of 288 cattle blood samples, collected from 6 provinces in China, were found to be positive. Among the positive samples, only 3 allelic MPSP gene types (Chitose [C]-, Ikeda [I]-, and buffeli [B]-type) were successfully amplified. Moreover, the results revealed that the majority of the parasites sampled in this study were C- and I-type (prevalence of 84 and 69%, respectively), whereas the B-type was less common (prevalence of 36%). Co-infections with C-, I-, and B-type T. sergenti also were found. An additional known allele, Thai-type, was not detected. Phylogenetic analysis based on the MPSP gene sequences, including 3 standard stocks generated in the laboratory (T. sergenti Wenchuan, T. sergenti Ningxian, and T. sergenti Liaoyang), revealed that the isolates of Chinese sergenti were comprised of at least 4 allelic MPSP gene types, i.e., C-, I-, B1-, and B2-type, and these parasites with 6 MPSP types 1–5 and 7 were present in China.

Ai Hong Liu, Gui Quan Guan, Jun Long Liu, Zhi Jie Liu, Neil Leblanc, You Quan Li, Jin Liang Gao, Mi Ling Ma, Qing Li Niu, Qiao Yun Ren, Qi Bai, Hong Yin, and Jian Xun Luo "Polymorphism Analysis of Chinese Theileria sergenti Using Allele-Specific Polymerase Chain Reaction of the Major Piroplasm Surface Protein Gene," Journal of Parasitology 97(1), 116-121, (1 February 2011). https://doi.org/10.1645/GE-2444.1
Received: 27 January 2010; Accepted: 1 August 2010; Published: 1 February 2011
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