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1 April 2011 Partial Sequence of the Alpha-Tubulin Gene From Histomonas meleagridis Isolates From the United States
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Abstract
Histomonas meleagridis, the causative agent of histomoniasis, is a protozoan parasite classified in the Dientamoebidae (order Tritrichomonadida). The α-tubulin gene of 7 H. meleagridis isolates originating from either domestic chickens or turkeys from the United States was amplified by nested PCR and sequenced. A 91.4–99.8% nucleotide identity was shared among the 7 different sequences, and phylogenetic analysis disclosed that the 7 isolates were divided into at least 3 clades. These sequences had a 91–99% nucleotide identity and a 96–100% amino acid identity compared with 3 H. meleagridis α-tubulin sequences obtained from isolates originating from turkeys in Germany. Further α-tubulin gene analysis from species in the Dientamoebidae will be useful in elucidating the evolutionary relationship of these protozoans.
Richard W. Gerhold, Lori A. Lollis, Larry R. McDougald and Robert B. Beckstead "Partial Sequence of the Alpha-Tubulin Gene From Histomonas meleagridis Isolates From the United States," Journal of Parasitology 97(2), (1 April 2011). https://doi.org/10.1645/GE-2618.1
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