Schistosomula, the larval stage of schistosomes in vertebrate hosts, are highly vulnerable and considered an ideal target for vaccine and drug development. Although the schistosomule stage is essential for biological studies, collecting sufficient numbers of schistosomula from their definitive hosts in vivo is difficult to accomplish. However, in vitro collection via cercariae transformation can effectively yield high numbers of schistosomula. We compared a current and widely used double-ended–needle mechanical transformation method to a culture medium based on a nonmechanical method. We found the rates of transformed cercariae, i.e., separated cercariae heads from tails, differed by only 2–7% at 0.5, 1, and 2 days in culture and that there was no significant difference in the number of transformed cercariae between the transformation methods at 3 and 4 days in culture. Notably, the mechanical and nonmechanical cercariae transformation methods both yielded significantly large and similar quantities of viable schistosomula. Given that the nonmechanical method is simpler and less damaging to the parasites, we recommend the use of it as an alternative way for in vitro cercariae transformation. In addition, we also observed morphological changes of the detached cercariae tails in culture medium. Interestingly, the tails are able to regenerate head-like organs/tissues and survive for at least 4 days. This intriguing change suggests unique biological features of the cells in the tails.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 98 • No. 6