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1 June 2013 Vaccination of Mice with an Antigenic Serine Protease-Like Protein Elicits a Protective Immune Response Against Trichinella spiralis Infection
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Abstract
Trichinellosis has major economic impacts on animal husbandry and food safety, and the control and elimination of trichinellosis is a major objective of veterinary medicine. A gene encoding serine protease of Trichinella spiralis (Ts-Adsp) was identified by immunoscreening an adult T. spiralis cDNA library. In this study, the recombinant Ts-Adsp protein (rTs-Adsp) was cloned and expressed in a prokaryotic expression system and purified by Ni-affinity chromatography. To determine whether the purified rTs-Adsp is a potential vaccine candidate for the control of T. spiralis infection, we immunized BALB/c mice with this protein in combination with an alum adjuvant and subsequently challenged with T. spiralis larvae. The results showed that mice vaccinated with rTs-Adsp exhibited an average reduction in the muscle larvae burden of 46.5% relative to the control group. Immunization with the rTs-Adsp antigen induced both humoral and cellular immune responses, which manifested as elevated specific anti–rTs-Adsp IgG and IgE antibodies and a mixed Th1–Th2 response, as determined by Th1 (IFN-γ and IL-2) and Th2 (IL-4, IL-10, and IL-13) cytokine profiling, with the Th2 predominant. Thus, purified rTs-Adsp is able to limit the invasion of T. spiralis, and this protein could be an effective vaccine candidate for trichinellosis.
Shuang Feng, Xiuping Wu, Xuelin Wang, Xue Bai, Haining Shi, Bin Tang, Xiaolei Liu, Yanxia Song, Pascal Boireau, Feng Wang, Ying Zhao and Mingyuan Liu "Vaccination of Mice with an Antigenic Serine Protease-Like Protein Elicits a Protective Immune Response Against Trichinella spiralis Infection," Journal of Parasitology 99(3), (1 June 2013). https://doi.org/10.1645/12-46.1
Received: 11 August 2012; Accepted: 1 December 2012; Published: 1 June 2013
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