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1 June 2013 Species-Specific Primers Designed from RAPD Products for Bithynia funiculata, the First Intermediate Host of Liver Fluke, Opisthorchis viverrini, in North Thailand
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Abstract

Bithynia funiculata is the first intermediate host of the human liver fluke Opisthorchis viverrini in northern Thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. As a consequence, we focused on the development of species-specific primers for use as an identification tool. Our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by analyzing specimens of B. funiculata from 3 locations in northern Thailand. This primer set did not amplify other species in the Bithyniidae or in other gastropod families. By providing a tool to confirm morphological identifications of B. funiculata, and by enabling the identification of juvenile specimens and those with damaged shells, these primers will improve estimates of the prevalence of parasitic infections in this snail.

Jutharat Kulsantiwong, Sattrachai Prasopdee, Supawadee Piratae, Panita Khampoosa, Apiporn Suwannatrai, Wipada Duangprompo, Thidarut Boonmars, Wipaporn Ruangjirachuporn, Jiraporn Ruangsittichai, Vithoon Viyanant, Paul D. N Hebert, and Smarn Tesana "Species-Specific Primers Designed from RAPD Products for Bithynia funiculata, the First Intermediate Host of Liver Fluke, Opisthorchis viverrini, in North Thailand," Journal of Parasitology 99(3), (1 June 2013). https://doi.org/10.1645/GE-3138.1
Received: 8 March 2012; Accepted: 1 November 2012; Published: 1 June 2013
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