The location of the homestay agglomeration is closely related to its proximity to the resources environment and the humanities and social environments. From the macroscopic and microscopic aspects, this study uses the analytic hierarchy process and the expert consultation method to construct a relatively comprehensive evaluation system including economic and environmental factors, tourism development factors, geographical environment factors, supporting facilities and operating conditions. The evaluation index system consists of 20 evaluation indexes and 35 evaluation factors. Taking the Baileqiao in Hangzhou and Qianjiadian Town in Beijing as examples, the evaluation indexes are verified and applied. The results show that the main factors affecting the location of the homestay agglomeration are the economic and environmental factors. The evaluation index system is composed of a target layer, a comprehensive evaluation layer, a factor evaluation layer and an index layer. The target layer takes reasonable location of the homestay agglomeration as the overall objective; the comprehensive evaluation layer includes economic and environmental factors (0.4396), tourism development (0.3039), geographic and environmental factors (0.1405), and supporting facilities (0.0545). The comprehensive evaluation scores of Baileqiao and Qianjiadian homestay agglomerations are 92.644 and 88.734, respectively, indicating that both are reasonable homestay sites. This study is helpful for promoting the understanding of the homestay agglomeration location selection and the development and construction of homestay agglomeration sites.