The concept of ecological security stems from environmental security theory, and is generally used to evaluate the ecological security of land, water resources and the city. This study applies the Pressure-Station-Response (P-S-R) model and theory of ecological security with field research in 2010 to establish an index system for tourism ecological security evaluation. The findings show that tourism ecological security in Wuhan has increasingly improved from a comprehensive evaluation value of 5.8023 in 2004 to 6.459 in 2010, and has moved from a “sensitive” condition to “safe” condition. Ecological pressure and ecological response are negatively correlated, and the value of ecological response was below that of ecological pressure during the seven years. In order to make clear the impact factors affecting tourism ecological security in Wuhan, this paper summarizes ten limiting factors influencing tourism ecological security and makes several recommendations to improve tourism ecological security.