A factorial experiment, in which blacklip (Haliotis rubra; Leach, 1814) and greenlip abalone eggs (H. laevigata; Donovan, 1808) were exposed to a range of conspecific sperm densities (ca. 104–107 sperm mL−1) for different time intervals (7–2,400 s), showed there was a significant interaction between these factors in both species. Prolonged exposure (i.e., 1,200–2,400 s for blacklips and 480–2,400 s for greenlips) to concentrated sperm (i.e., 107 sperm mL−1) resulted in lysis of the egg membrane and polyspermy. Analysis of CoVariance of F50 values (i.e., the sperm concentration required for 50% fertilization, derived from the linear regression of logit (proportion of eggs fertilized) versus sperm density) between species across a range of contact times demonstrated that contact time had a significant effect (P < 0.001) whereas species did not (P = 0.22). The lack of a species effect suggests that the fertilization potential of blacklip and greenlip abalone eggs are similar, at least across the range of sperm densities and contact times used. An examination of sperm morphology using scanning electron microscopy of both species revealed similarities in sperm length (i.e., 42–46 μm) and differences in the shape of acrosome, the tip of which was blunt in blacklip sperm and V-shaped in greenlip sperm. Morphological differences in haliotid sperm are discussed in relation to species differences in fertilization kinetics.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.