Translator Disclaimer
1 December 2009 Improved Methodology for Tracking and Genetically Identifying the Softshell Clam Mya arenaria
Author Affiliations +
Abstract

Attempts to characterize the population dynamics of the softshell clam Mya arenaria are complicated by a lack of nonlethal genotyping techniques and reliable tagging methods. A straightforward and nonlethal technique for clam genotyping is presented here, as well as a new method for the long-term tagging of clams. Hemolymph extracted from M. arenaria was used directly in a polymerase chain reaction to amplify successfully a DNA fragment suitable for sequencing. Tested M. arenaria showed 100% (n = 10) survival after a period of 4 wk. In a separate experiment, passive integrated transponder (PIT) tags were inserted between the mantle and shells of 72 clams that were monitored for tag retention and mortality. Among all PIT-tagged clams, there was 100% survival and 92% tag retention. These methods provide a mechanism by which softshell clams can be genotyped and individually monitored during field experiments.

Scott Hamilton and Laurie Connell "Improved Methodology for Tracking and Genetically Identifying the Softshell Clam Mya arenaria," Journal of Shellfish Research 28(4), 747-750, (1 December 2009). https://doi.org/10.2983/035.028.0402
Published: 1 December 2009
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top