Poor nutritional condition of seed of the green-lipped mussel Perna canaliculus (Gmelin 1791) is a major cause of significant losses after seeding out onto mussel farms. Biochemical analysis of experimentally unfed seed mussels showed they preferentially depleted glycogen reserves versus lipid and protein during short periods of starvation (≤8 days). This knowledge allowed the use of a human diabetic glucose meter to assess quickly the levels of glycogen in seed mussels after they were first homogenized and then digested with α-amylase, which converted the glycogen to glucose and maltose. The assay was sensitive enough to detect significant differences between mussels that had been continuously fed (6.04 ± 0.24 (SE) mmol/L) compared with mussels that had been unfed for 4 days (1.71 ± 0.06 mmol/L) or 8 days (0.67 ± 0.02 mmol/L). These results were consistent with biochemical analysis of the actual glycogen content of the experimental groups (16.6 ± 1.8 mg/g dry mass vs. 6.2 ± 1.0 mg/g and 5.5 ± 0.7 mg/g, respectively). This novel assay has the potential for quickly assessing the nutritional quality of seed mussels, enabling the reduction of losses of seed in the mussel farming industry.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 30 • No. 1