Translator Disclaimer
1 December 2011 Quantification of Manila Clam Ruditapes philippinarum (Adams & Reeve, 1850) Larvae Based on SYBR Green Real-Time Polymerase Chain Reaction
Author Affiliations +
Abstract
Biological and ecological research is seriously handicapped because of difficulties experienced in the reliable detection and quantification of bivalve larvae. This is a critical issue in the case of the Manila clam Ruditapes philippinarum (Adams & Reeve, 1850), a largely invasive and commercially relevant species, with important wild, cultured, and naturalized populations throughout the world. A SYBR Green real-time PCR assay, containing TPHI16S1F and TPHI16S2R primers (specific to R. philippinarum female mtDNA), was designed and tested to provide a rapid and high-throughput PCR-based method for larval quantification. Accurate estimations of larval numbers in spiked plankton samples point to the usefulness of this system. It can be used through a wide range of evaluated variable experimental conditions, such as the presence of closely related bivalve species, stationary-dependent plankton abundance, sampling volumes, and larval size.
Javier Quinteiro, L. Pérez-Diéguez, A. Sánchez, R. I. Perez-Martín, C. G. Sotelo and M. Rey-Méndez "Quantification of Manila Clam Ruditapes philippinarum (Adams & Reeve, 1850) Larvae Based on SYBR Green Real-Time Polymerase Chain Reaction," Journal of Shellfish Research 30(3), (1 December 2011). https://doi.org/10.2983/035.030.0319
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top