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1 April 2012 Identification of an Aflp Fragment Linked to Shell Color in the Noble Scallop Chlamys nobilis Reeve
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Abstract
The DNA amplified fragment length polymorphism (AFLP) fingerprinting approach was used to identify a shell color-linked marker in the noble scallop Chlamys nobilis Reeve. The marker B1f176 was linked completely to the gene for bright-orange shell color with no recombination in the progeny we sampled. This marker was amplified in bright-orange parents and all orange progeny, but was absent in all of the brownish purple progeny. Nevertheless, AFLP markers are not feasible for large-scale application. Thus, we converted B1f176 into a sequence-characterized amplified region (SCAR) marker that we designated as SCAR Orange-142. The marker Orange-142, like the AFLP band from which it was derived, amplified a robust band in only the bright-orange scallops, both in F1 and F2 families as well as in a test group obtained from a seafood market. These results indicate that Orange-142 is tightly linked to the gene for orange shell color. In all but 1 of 10 F2 families derived from bright-orange F0 grandparents, the observed frequencies of orange progeny were consistent with Mendelian ratios expected from a 1-locus 2-allele system in which the brownish purple genotype was recessive. This study is the first report of the genetic basis for shell color in C. nobilis. The identification of a shell color-linked marker in the noble scallop improves our understanding of shell color inheritance and may contribute to C. nobilis broodstock enhancement programs using marker-assisted selection.
Tao Yuan, Maoxian He and Liangmin Huang "Identification of an Aflp Fragment Linked to Shell Color in the Noble Scallop Chlamys nobilis Reeve," Journal of Shellfish Research 31(1), (1 April 2012). https://doi.org/10.2983/035.031.0104
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