Translator Disclaimer
1 August 2012 Cloning and Expression Analysis of Allograft Inflammatory Factor Type 1 in Coelomocytes of Antarctic Sea Urchin (Sterechinus neumayeri)
Author Affiliations +
Abstract
We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival.
Fernanda Ovando, Carla Gimpel, Constanza Cardenas, Jose Roberto Machado Cunha Da Silva, Julien De Lorgeril and Marcelo Gonzalez "Cloning and Expression Analysis of Allograft Inflammatory Factor Type 1 in Coelomocytes of Antarctic Sea Urchin (Sterechinus neumayeri)," Journal of Shellfish Research 31(3), (1 August 2012). https://doi.org/10.2983/035.031.0336
JOURNAL ARTICLE
9 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top